Fig. 3

Interactions between PNS and transplanted melanoma cells in larval zebrafish.

A Representative images of a 5 dpf tg(nbt:dsRed) larva, acquired with a fluorescent microscope. Zoomed area: picture of the same larvae acquired with a confocal microscope. Scale bar = 250 μm. White arrowheads = enteric neural cell bodies. White asterisks = axons. B Representative images of xenografted larvae with A375P cells on D2, acquired with a confocal microscope. Transplanted A375P cells are in green, neurons are in red. A375 cells seem to escape from the primary cancer mass by following the axons as a route of dissemination (white arrowheads). First column: z-projection images (z-stack = 170–200 μm) of larva 1 and larva 2. Scale bar = 250 μm. Second and third columns: zoomed images of the region around the SB of larva 1 and larva 2 corresponding to different single slices. Scale bar = 50 μm.