FIGURE

Fig. 3

ID

ZDB-FIG-250526-62

Publication

Shi et al., 2025 - Gastrodin promotes CNS myelinogenesis and alleviates demyelinating injury by activating the PI3K/AKT/mTOR signaling

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Fig. 3

Gastrodin promotes OL maturation in vitro.

a Schematic depicting OPCs culture and gastrodin treatment. b Western blot for MBP after 1, 10, 50 μM gastrodin treatment. c Quantification of relative MBP protein levels (n = 3 experiments). d Representative images of PDGFRα (red), CC1 (green), and Olig2 (blue) immunofluorescence and quantification of proportion of CC1⁺ cells or PDGFRα⁺ cells in Olig2⁺ cells (n = 3 experiments). e Representative images of OLs membrane area of MBP⁺ cells, depicting different degrees of OL maturation. Low differentiation, OLs without membrane; Medium differentiation, OLs with a few membranes; High differentiation, OLs with large area of membrane. f Representative images of MBP (red) and Sox10 (green) immunofluorescence. White boxes zoom in are shown on the right. g Quantification of MBP⁺ cells’ proportion in different maturation degrees (n = 3 experiments). h Quantification of MBP⁺ area (n = 3 experiments). i Quantification of proportion of MBP⁺ cells in Sox10⁺ cells (n = 3 experiments). j Schematic depicting MO3.13 oligodendroglial cell line culture and gastrodin treatment. k qPCR analysis of Mbp and Plp1 after 10 and 50 μM gastrodin treatment (n = 3 experiments). Data are represented as mean ± SEM, One-way ANOVA and Dunnett-t in c, unpaired Student’s t test in d, g–i, and k.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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