FIGURE

Fig. 5

ID

ZDB-FIG-250507-20

Publication

Griffin et al., 2025 - Retinoic acid promotes second heart field addition and regulates ventral aorta patterning in zebrafish

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Fig. 5

The SHF progenitor pool is present, and apoptosis is not increased in late RA-deficient embryos. (A?B) ltbp3 expression in embryos treated with DMSO or DEAB from 26 hpf to 33 hpf. (C, D) Activated Caspase-3 distribution (green) at 32 hpf in the heart (indicated by MF20 staining in red) and adjacent regions in embryos treated with DMSO (n = 26/28 with no staining in heart) or DEAB (n = 27/29 with no staining in heart). White ovals outline the heart region, V indicates ventricle region of heart tube. (E, F) Activated Caspase-3 distribution at 48 hpf in the heart and adjacent regions in embryos treated with DMSO (n = 21/21 with no staining in the heart) or DEAB (n = 16/18 with no staining in the heart). (G, H) Activated Caspase-3 distribution at 72 hpf in the heart and adjacent regions in embryos treated with DMSO (n = 31/32 as shown) or DEAB (n = 31/32 as shown). White circles outline the ventricle and OFT region in E-H. Maximum intensity projections of a Z-stack are shown for each image. Anterior is to the left. Scale bars are 50 ?M. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, , Griffin, A.H.C., Small, A.M., Johnson, R.D., Medina, A.M., Kollar, K.T., Nazir, R.A., McGuire, A.M., Schumacher, J.A., Retinoic acid promotes second heart field addition and regulates ventral aorta patterning in zebrafish, , Copyright (2025) with permission from Elsevier. Full text @ Dev. Biol.