Fig 5

Transcriptomics profiling of slc13a5 mutants.

(A-B) Bulk RNA sequencing was performed on pools of zebrafish heads of 5 dpf WT, 5a^−/−, 5b^−/− and 5a^−/−;5b^−/−. WT, 5a^−/−, 5b^−/− and 5a^−/−;5b^−/−, n =  3 ×  15 heads pooled. Heatmaps were constructed for prioritized DEGs using ComplexHeatmap. Genes upregulated in 5a^−/− and 5a^−/−;5b^−/− larvae compared to WTs (padj < 0.05 and log2FC >  1.5) are involved in apoptosis, inflammation and immune response and metabolism (A). Genes upregulated in 5b^−/− larvae compared to WTs (padj < 0.05 and log2FC >  1.5) are involved in apoptosis and inflammation and immune response (A). Genes downregulated in 5a^−/− and 5a^−/−;5b^−/− larvae compared to WTs (padj < 0.05 and log2FC < −1.5) are involved in epilepsy and other neurological disorders, calcium ion transport, sleep regulation and negative regulation of apoptosis (B). Genes downregulated in 5b^−/− larvae compared to WTs (padj < 0.05 and log2FC < −1.5) are involved in neurological disorders and negatively regulate apoptosis (B). Heatmaps with a full list of DEGs are provided in S7–S10 Figs. DEGs, differentially expressed genes. The data underlying this figure can be found in S2 Data.