Fig. 8

Mmp14b is essential for macrophage presence and ECM remodeling at the border zone.

A Collagen hybridizing peptide (CHP) and mScarlet immunostaining in Tg(mpeg1:EGFP); Tg(myl7:lck-mScarlet) ventricles from mmp14b^Δ/Δ and wild-type siblings at 10 dpci. White dashed lines indicate the wound border and yellow boxes contain zoomed images from the wound border zone. B Quantification of CHP intensity at the border zone (arb. units, arbitrary units) in Tg(mpeg1:EGFP); Tg(myl7:lck-mScarlet) ventricles from mmp14b^Δ/Δ (n = 5 ventricles) and wild-type siblings (n = 4 ventricles) at 10 dpci. Data are presented as mean ± SD. P-value was calculated using an unpaired two-sided t-test. Source data are presented in the Source Data file. C GFP and mScarlet immunostaining in Tg(mpeg1:EGFP); Tg(myl7:lck-mScarlet) ventricles from mmp14b^Δ/Δ and wild-type siblings at 10 dpci. White dashed lines indicate the approximate wound border. D Quantification of mpeg1:EGFP+ cells 50 μm proximal and distal to the wound border in ventricles from mmp14b^Δ/Δ (n = 6 ventricles) and wild-type siblings (7 dpci n = 7 ventricles, 10 dpci n = 6 ventricles) at 7 and 10 dpci. Data are presented as mean ± SD. P-values were calculated using unpaired two-sided t-tests and corrected for multiple comparisons using the Holm-Sidak method. Source data are presented in the Source Data file. E RT-qPCR analysis of fibroblast marker genes in mmp14b^Δ/Δ mutant (n = 5 ventricles) and wild-type sibling (n = 5 ventricles) at 10 dpci. Data are presented as mean ± SD. P-values were calculated using an unpaired two-sided t-test or a two-sided Mann–Whitney test (col1a1a). Source data are presented in the Source Data file. Fcol12a1a in situ hybridization chain reaction (HCR) in mmp14b^Δ/Δ mutant and wild-type sibling ventricles at 10 dpci. Yellow boxes denote zoomed images at the cortical BZ, blue boxes denote zoomed images at the apex of the wound. Epi, epicardium. Scale bars: 100 μm, 20 μm in the zoomed images in (F).