Fig. 4

A Immunostaining of GFP and F-actin in Tg(mpeg1:EGFP) ventricles from irf8^st96/st96 mutants and wild-type siblings at 10 dpci. White dotted line denotes the approximate wound border. B Quantification of mpeg1:EGFP+ cells 50 μm proximal and distal to the wound border in Tg(mpeg1:EGFP) ventricles from irf8^st96/st96 mutants and wild-type siblings at 4 (n = 4 wild-type, n = 4 mutant ventricles), 7 (n = 5 wild-type, n = 5 mutant ventricles), and 10 (n = 4 wild-type, n = 5 mutant ventricles) dpci. Data are presented as mean ± SD. P-values were calculated using multiple unpaired two-sided t-tests and corrected for multiple comparisons using the Bonferroni-Dunn method. Source data are presented in the Source Data file. C Phalloidin staining of F-actin in thick cryosections of zebrafish ventricles from irf8^st96/st96 mutants and wild-type siblings at 10 dpci (left). Quantification of the number of CM protrusions per 100 micron of wound border (middle) and length of CM protrusions (right) from thick cryosections of irf8^st96/st96 mutant and wild-type sibling ventricles at 10 dpci (n = 485 CM protrusions from 6 wild-type ventricles, n = 240 CM protrusions from 5 mutant ventricles). Data are presented as mean ± SD (number of CM protrusions) and violin plots of all points with solid gray lines indicating the median and dotted gray lines indicating 25th and 75th percentile (length of CM protrusions). P-values were calculated using an unpaired two-sided t-test (number of CM protrusions) and a two-sided Mann–Whitney test (CM protrusion length). Source data are presented in the Source Data file. D Schematic illustrating the experimental set-up to ablate macrophages using clodronate liposomes (CL). Created in BioRender. Beisaw, A. (2025) https://BioRender.com/2iptntt. E Phalloidin staining of F-actin in thick cryosections of zebrafish ventricles treated with PBS liposomes or clodronate liposomes at 10 dpci. F Quantification of the number of CM protrusions per 100 micron of wound border (left) and length of CM protrusions (right) from thick cryosections of ventricles from PBS (n = 521 CM protrusions from 4 ventricles) and CL (n = 664 CM protrusions from 5 ventricles) treated fish at 10 dpci. Data are presented as mean ± SD (number of CM protrusions) and violin plots of all points with solid gray lines indicating the median and dotted gray lines indicating 25th and 75th percentile (CM protrusion length). P-values were calculated using an unpaired two-sided t-test (number of CM protrusions) and a two-sided Mann–Whitney test (CM protrusion length). Source data are presented in the Source Data file. PBS PBS liposomes, CL clodronate liposomes. Scale bars: 100 μm.