FIGURE

Fig. 3

ID

ZDB-FIG-250423-75

Publication

Wu et al., 2025 - KDM4C works in concert with GATA1 to regulate heme metabolism in head and neck squamous cell carcinoma

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

The interaction of KDM4C and GATA1. A Association of ectopically expressed KDM4C and GATA1. Western blot analysis of HEK293T cells co-transfected with pcDNA3.1-HA-KDM4C and pcDNA3.1-myc-his-GATA1 as indicated. Immunoprecipitation (IP) was conducted using anti-HA or anti-myc antibody. Rabbit/mouse IgG (1:1) was used as a negative control. B Western blot analysis of endogenous IP in SAS cells. IP was conducted using anti-KDM4C or anti-GATA1 antibody. Rabbit IgG was used as negative control. C Co-IP assays were performed with an anti-myc antibody in lysates from HEK293T cells co-transfected with a full-length (FL) myc-GATA1 vector plus one of the HA-tagged constructs [mock, full-length (FL) KDM4C, or KDM4C truncated mutants (∆N135, ∆N490 and ∆C185)], followed by western blot analysis. D Co-IP assays were performed with an anti-HA antibody in lysates from HEK293T cells co-transfected with a full-length (FL) HA-KDM4C vector plus one of the myc-tagged constructs [mock, full-length (FL) GATA1, or GATA1 truncated mutants (∆C170, ∆N199 and ∆N249)], followed by western blot analysis

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell. Mol. Life Sci.