ZFIN Image: Wu et al., 2025, Fig. 3

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Figure Caption

Fig. 3

The interaction of KDM4C and GATA1. A Association of ectopically expressed KDM4C and GATA1. Western blot analysis of HEK293T cells co-transfected with pcDNA3.1-HA-KDM4C and pcDNA3.1-myc-his-GATA1 as indicated. Immunoprecipitation (IP) was conducted using anti-HA or anti-myc antibody. Rabbit/mouse IgG (1:1) was used as a negative control. B Western blot analysis of endogenous IP in SAS cells. IP was conducted using anti-KDM4C or anti-GATA1 antibody. Rabbit IgG was used as negative control. C Co-IP assays were performed with an anti-myc antibody in lysates from HEK293T cells co-transfected with a full-length (FL) myc-GATA1 vector plus one of the HA-tagged constructs [mock, full-length (FL) KDM4C, or KDM4C truncated mutants (∆N135, ∆N490 and ∆C185)], followed by western blot analysis. D Co-IP assays were performed with an anti-HA antibody in lysates from HEK293T cells co-transfected with a full-length (FL) HA-KDM4C vector plus one of the myc-tagged constructs [mock, full-length (FL) GATA1, or GATA1 truncated mutants (∆C170, ∆N199 and ∆N249)], followed by western blot analysis

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