Fig. 6

FASTFUCCI, a green-NIR fluorescent chemogenetic cell-cycle indicator.a HEK293T cells expressing FASTFUCCI and treated with 1 µM HMBR (to assemble pFAST540) and 10 µM HPAR-3,5DOM (to assemble nirFAST715) were imaged by timelapse confocal microscopy (1 frame every 20 min) for 24 h. Relevant timepoints of the designated field of view are shown to highlight transitions between the different cell cycle stages. Representative micrographs from three independent experiments (see also Supplementary Movie 4). b Fluorescence intensity evolution of tracked cell (cell A), which upon mitosis gives cells A’ and cells A”, is shown over time. Cell tracking and lineage was achieved through the Trackmate plugin (Fiji)41,42. See Supplementary Table 8 for detailed imaging settings. Source data are provided as a Source Data file.