Fig. 7

Mitochondrial oxidative stress induces lipid peroxidation in cystinosis podocytes. (a, b, c, d) Ratio of the percentages of BODIPY-C11?+?cells measured by flow cytometry incubated with 10 µM cysteamine, 10 µM MitoTEMPO, or 100 µM cysteamine?+?10 µM MitoTEMPO for 48 h, normalized to a. CTNS?/?#1, b. CTNS?/?#2, c. shCTNS#1, and d. shCTNS#2. (n???3 biological experiments, n?=?1 technical replicate). Statistical analysis: Dunnett?s One-Way ANOVA. (e, f) Ratio of the percentages of BODIPY-C11?+?cells among dead podocytes (Zombie) measured by flow cytometry incubated with 100 µM cysteamine, 10 µM MitoTEMPO, or 100 µM cysteamine?+?10 µM MitoTEMPO for 48 h, normalized to e. CTNS?/?#1 and f. CTNS?/?#2. (n???3 biological experiments, n?=?1 technical replicate). Statistical analysis: Dunnett?s One-Way ANOVA. (g, h, i, j) Ratio of the percentages of BODIPY-C11?+?cells measured by flow cytometry incubated with 10 µM liproxstatin-1 or 100 µM cysteamine?+?10 µM liproxstatin-1 for 48 h, normalized to g. CTNS?/?#1, h. CTNS?/?#2, i. shCTNS#1, and j. shCTNS#2. Data for the CTNS?/?#1, CTNS?/?#2, shCTNS#1, and shCTNS#2 groups are the same as those in (A-D), respectively. (n???3 biological experiments, n?=?1 technical replicate). Statistical analysis: Dunnett?s One-Way ANOVA. (k, l) Ratio of the percentages of BODIPY-C11?+?cells among dead podocytes (Zombie) measured by flow cytometry incubated with 10 µM liproxstatin-1 or 100 µM cysteamine?+?10 µM liproxstatin-1 for 48 h, normalized to k. CTNS?/?#1 and l. CTNS?/?#2. Data for the CTNS?/?#1 and CTNS?/?#2 groups are the same as those in (e?f), respectively. (n???3 biological experiments, n?=?1 technical replicate). Statistical analysis: Dunnett?s One-Way ANOVA. Each dot represents a biological experiment