FIGURE

Fig. 1

ID

ZDB-FIG-250303-1

Publication

Escot et al., 2025 - Nance-Horan-syndrome-like 1b controls mesodermal cell migration by regulating protrusion and actin dynamics during zebrafish gastrulation

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Fig. 1

nhsl1b is expressed in mesodermal cells during gastrulation.

A Whole-mount in situ hybridisation with nhsl1b or control probes, on embryos fixed at the 1-cell, shield (onset of gastrulation), 75% epiboly (mid-gastrulation) and 12-somite stages. B, C Whole mount in situ hybridisation with nhsl1b and sox17 probes and subsequent GFP immunostaining. Bnhsl1b is expressed in induced mesendodermal cells. N = 4 experiments. C No expression of nhsl1b is observed in induced endodermal cells. N = 3 experiments. D Whole-mount double in situ hybridisation with sox17 (red) and nhsl1b (green) probes at 75% epiboly (end of gastrulation) and bud stages. nhsl1b is not expressed in the sox17 expressing fore-runner cells. Scale bar 100 µm unless specified.

Expression Data

Genes:	nhsl1b sox17
Fish:	AB
Anatomical Terms:	axial chorda mesoderm forerunner cell group hypoblast margin mesodermal cell somite whole organism
Stage Range:	1-cell to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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