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nhsl1b overexpression affects lateral mesodermal migration, reducing cell speed and persistence. A Representative lateral views of Tg(tbx16:EGFP) embryos, at early gastrulation (60% epiboly; t = 0) and 1 hour later, in embryos injected with lacZ (control, n = 6) or nhsl1b (n = 10) mRNAs. Dashed lines indicate the positions of the front of the migrating mesoderm on the first (yellow) and last (white) frames. Mesoderm progression was measured as the distance between these two lines (see also Supplementary Fig. S1). Scale bar 100 µm. B Quantification of the lateral mesoderm progression in control and nhsl1b mRNA injected embryos. N = 6 experiments. Mann–Whitney. p-value: Control vs Nhsl1b: 0.0075*, C, D Instant cell speed and directionality ratio. Circles on the violon plot indicate mean per embryo. Likelihood ratio test of a linear mixed effects model with treatment as a fixed effect and embryos as a random effect against a model without the fixed effect. p-values: 0.0029 ** and 0.0111 *. E Directional autocorrelation. Likelihood ratio test of a non-linear mixed effects model (see methods) with treatment as a fixed effect and embryos as a random effect against a model without the fixed effect. p-value: 0.03589 *. Control (n = 4) and nhsl1b (n = 10) mRNA injected embryos.
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