Fig. 2

Design and synthesis of host–guest supramolecular fluorescent chemodosimeters for selective and rapid response toward EP.

a Schematics of guest recognition molecules G1–G3 with varies alkyl chain lengths, host molecules CB[n]s with different cavity sizes, and neurotransmitters to be tested. b The self-assembly of the developed and optimized CMG2 supramolecular fluorescent sensor for EP. c Fluorescence titration spectra of MB (10 μm) toward addition of CB¹⁰-G2 (concentration ratio, 1:1) with different concentration (0−15 μm) in PBS buffer (10 mm, pH = 7.4) containing 0.05% DMSO; Illustration: CB¹⁰-G2 with MB assembled molar ratio. Data are presented as mean ± S.D. Error bars: S.D., n  =  5 independent experiments. d Interference of G1, G2, G3, CM8G2 and CMG2 on NE and DA (Set the response value of CMG2 for EP to 1.0). Data are presented as mean ± S.D. Error bars: S.D., n  =  5 independent experiments. e Kinetic fluorescence responses of the G1–G3 (each 10 μm) and the assembled host-guest supramolecular fluorescent sensor toward addition of 5 μm EP in PBS (10 mm, pH 7.4). f Reaction rate constants (k_obs) were measured for the G1–G3 and the assembled host-guest supramolecular fluorescent sensor in response to EP. The pseudo-first-order rate constants (k_obs) obtained from the slopes of plot of ln[(F_max–F_t)/F_max] vs time. F_max is the maximum fluorescence intensity during the measurement time, F_t is the fluorescence intensity at the corresponding time points (F_max, F_t: The fluorescent emission wavelength is 570 nm). g The molecular structure of CMG2 after DFT optimization and the quantification of the distance between two recognition sites of G2. The above-mentioned source data are provided as a Source Data file.