FIGURE

Fig 6

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ZDB-FIG-250207-66

Publication

Widziolek et al., 2025 - Gingipains protect Porphyromonas gingivalis from macrophage-mediated phagocytic clearance

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Fig 6

Flow cytometry analysis of phagocytosis of Pg W83 and ΔK/R-ab mutant by macrophages and neutrophils in zebrafish larvae.

Transgenic zebrafish larvae Tg(mpeg1:mCherry) x Tg(mpx:EGFP) were infected with AF647-SE-stained Pg W83 or ΔK/R-ab and flow cytometry analysis was performer 24 hpi. Gating strategy used for flow cytometry analysis from zebrafish larval homogenate (A). Gating strategy used to enumerate population of EGFP+ neutrophils (B-E) or mCherry+ macrophages (F-I) 24 hpi. Percentage of neutrophils or macrophages associated with bacteria (J). Mean fluorescence intensity of neutrophils and macrophages associated with bacteria (K). Representative confocal microscopy image of cells sorted from infected larvae (L). Red–macrophages, green—neutrophils, yellow- Pg. hpi–hours post infection.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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