Fig 1

Wild-type Porphyromonas gingivalis (Pg) W83 strain displays greater levels of survival in systemically infected zebrafish larvae compared to gingipain-null ΔK/R-ab mutant.

Zebrafish embryos were infected systemically at 30 hpf with fluorescein-SE-labelled wild-type Pg W83 or the gingipain-null mutant ΔK/Rab. PBS was injected into control larvae. (A) Representative images of real-time light-sheet microscopy over 16 h showing the differences in the course of bacterial eradication. White arrowheads point to Pg localised in puncta. Scale bar = 100 μm. (B) Zebrafish larvae were infected with Pg W83 or ΔK/R-ab, and larvae were homogenized at indicated time post-infection (hpi), serially diluted and number of recovered bacteria were enumerated on ABA plates. CFU–colony forming unit. Data are presented as means ± SEM. Each dot represents the number of bacteria recovered from an individual larva. Statistical significance was analysed by Mann-Whitney test, *** p≤0.001, **** p ≤ 0.0001.