Fig 4

Inactivation of gingipains by KYT inhibitors increases larvae survival and increases phagocytosis of wild-type W83 Porphyromonas gingivalis (Pg).

(A) Kaplan-Meier survival plot of zebrafish larvae (30 hpf) infected with wild-type Pg W83 pre-treated or untreated with KYT inhibitors. Comparisons between survival curves were made using the log rank test. *p≤0.05,***p≤0.001; hpi- hours post infection. Real-time analysis of the acidification process during phagocytosis of the Pg W83 treated/untreated with KYT inhibitors in the yolk (B) and tail (C) regions 2 hpi. Pg were stained with fluorescein-SE (green) and pHrodo (red). White dashed lines indicate yolk or tail areas. Boxes show zoomed regions of the images. y-yolk, h-heart. Scale bar = 100 μm. Quantified data from the yolk (D) and the tail (E) regions. Data are presented as means ± SD. Each dot represents signal quantified from a single larva. Statistical significance was analysed by unpaired t-test with Welch’s correction. **p≤0.01.