FIGURE

Fig. 5.

ID

ZDB-FIG-241218-50

Publication

Desingu Rajan et al., 2024 - Generation of a zebrafish neurofibromatosis model via inducible knockout of nf2

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Fig. 5.

nf2a/b knockout leads to Schwann cell hyperplasia. (A) Schematic representing the experimental strategy. (B-I) Confocal images of the pLLn SCs of Cas9-only controls (B-E) and nf2a/b knockout (F-I) embryos at 3 dpf and 5 dpf. pLLn SCs are labeled by Tg(sox10:mRFP) (magenta) and counterstained by DAPI (gray). Images represent confocal maximum-intensity projections of whole-mount embryos. (J,K) Bar plots showing quantification of the Sox10-positive pLLn SCs at 3 dpf (J) and 5 dpf (K) (ns, not significant; ****P<0.0001; each dot represents data from one larva). Each dataset was compared using unpaired two-tailed Student's t-test in GraphPad Prism. Error bars indicate s.e.m. across n=10 embryos. Each dot represents average of counts from three regions of pLLn SCs per embryo.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.