FIGURE

Fig. 4.

ID

ZDB-FIG-241218-49

Publication

Desingu Rajan et al., 2024 - Generation of a zebrafish neurofibromatosis model via inducible knockout of nf2

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Fig. 4.

nf2a/b knockout leads to increased meningeal proliferation. (A) Schematic representing the experimental strategy. Blue dashed line box in the 3 dpf zebrafish schematic indicates the imaged region. (B-G) Confocal images of the cranial region of 3 dpf Cas9-only (B-D) and nf2a/b knockout (E,F,G) embryos show the overlap of meningeal marker igfbp2a and proliferation marker pH3. (E′,F′,G′) Yellow dashed line boxes show the magnified region of the overlap in the nf2a/b knockout embryos. (H) Bar plot showing quantification of normalized proliferation (*P=0.0197; each dot represents data from one larva). Normalized proliferation=Igfbp2a and pH3 double-positive cells/total pH3-positive cells in the analyzed region. Each dataset was compared using unpaired two-tailed Student's t-test in GraphPad Prism. Error bars indicate s.e.m. across n=7 embryos. White dashed line marks the boundary between the brain and meninges. Images in B-E, F and G represent maximum-intensity projections of confocal planes in whole-mount embryos. E′, F′ and G′ represent confocal planes of 3.2 μm thickness. DAPI (gray) was used as a counterstain. b, brain; KO, knockout; pH3, phospho-histone 3.

Expression Data

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.