Figure 2

InP/ZnS QDs embryonic exposure caused retinal degeneration in zebrafish. a) Schematic illustration of the experimental design. b) Representative images of the distribution of InP/ZnS QDs on zebrafish eye using fluorescence microscopy. Scale bar, 200 µm. c) Determination of indium element content in the retina of zebrafish by ICP‐MS, n = 6, ND: No Detected. d) Representative images of zebrafish from 72 to 168 hpf after exposure. Scale bar, 1 mm. e) Schematic diagram of horizontal (X) and vertical (Y) axes of zebrafish eye. f) The lengths of X and Y axes in the zebrafish eye at 72–168 hpf, n = 20. Representative images of zebrafish retinal histology at 72 hpf g) and 168 hpf h) as assessed by HE staining. Scale bar, 50 µm. i‐j) Representative images of the retinas of Sofa zebrafish at 72 hpf i) and 168 hpf j) using a light‐sheet microscope. The retinal ganglion cell layer (GCL) is labeled by RPF, the inner nuclear layer (INL, composed of AC and HC) is labeled by GFP and CFP, BC is labeled by CFP, and the outer nuclear layer (ONL, composed of PR) labeled by RFP and CFP. k) Scheme of zebrafish eye. l) The representative images of zebrafish eye by optical coherence tomography (OCT) at 21 dpf. The data are presented as mean ± SE. One‐way ANOVA followed by Duncan test was used to assess the statistical significance. Significant change among different groups (p < 0.05) is indicated by the different letters on the bar.