Fig 3

Quantification of fasting glucose, random glucose, and insulin levels.

(A) Box plots showing (Aa,b) the amount of glucose (in μM) following the (Aa) fasting or (Ab) random glucose assay, and (Ac) the amount of insulin (in μl/ml), in larvae fed with the standard controls (SD), excess diet (ED), high fat diet (HFD) or SD + glucose diet (SDG), at 5 dpf, 6 dpf or 7 dpf. All the assays were repeated 9 times with n = 10 larvae each time. Medians are shown by horizontal lines inside the boxes, the 25^th and 75^th percentiles are shown as the bottom and tops of the boxes, and the minimum and maximum values are shown as the small horizontal lines below and above the boxes, and mean values are indicated as “+” sign. Significant differences relative to the SD controls are shown as *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001, as determined by two-way ANOVA followed by the Dunnett comparison test. (B) Representative fluorescence images of the localization of insulin in the pancreas of (Ba) SD, (Bb) ED, (Bc) HFD, and (Bd) SDG larvae, which were fixed at 7 dpf and immunolabeled with an anti-insulin antibody. Scale bar: 50 μm.