Fig. EV3

Knockdown of scar-6 shows no significant phenotype. (A) Schematic of antisense splice blocking morpholino oligo targeting the exon 1 splicing junction of scar-6 lncRNA. (B) Relative fold change expression of scar-6 upon of knockdown with splice blocking morpholino in zebrafish at 3 dpf. Data from 3 independent biological replicates plotted as mean fold change ± standard deviation; *P < 0.05 (two-tailed unpaired t-test). (C) Box and whisker plot representing the percentage of animals with haemorrhage phenotype in zebrafish embryos injected with 250 ?M of morpholino. Data from 4 independent experiments are represented by boxes indicating the interquartile range (25th to 75th percentiles), with the horizontal line within each box denoting the median. Whiskers extend to 1.5 times the interquartile range to define the minimum and maximum values, while individual points represent data from each experiment.: ns, not significant (two-tailed unpaired t-test). (D) Representative image of gib004Tg(fli1a:EGFP;gata1a:DsRed) zebrafish injected with different concentration of morpholino at 3 dpf. Red arrowhead denotes hemorrhage in the animals. 2.5× magnification, scale bar = 500 ?m. (E) Relative fold change expression of f10 and prozb upon knockdown of scar-6 lncRNA with splice blocking morpholino in zebrafish. Data from 3 independent biological replicates plotted as mean fold change ± standard deviation; ns, not significant (two-tailed unpaired t-test). (F) Coagulation assay plot calculating the time of occlusion (s) in different individual zebrafish in control and 500uM morpholino injected zebrafish. Each point represents the occlusion time of individual zebrafish segregated based on genotype; *P < 0.05 (Mann-Whitney U).