FIGURE

Fig. 6

ID

ZDB-FIG-241112-16

Publication

You et al., 2022 - Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

HAX1 promotes protein translation in HUVECs. (a) Analyses of nascent protein synthesis labeled with L-Homopropargyl glycine incorporation, detected in HUVECs-NC and HUVECs-shHAX1 by fluorescence microscopy. Quantify nascent protein expression by the quantification of fluorescence intensity. Blue: nuclei; Red: nascent protein, bar: 100 �m. (*p < 0.05, Student's t-test). (b) Polysome profiling of HUVECs-NC and HUVECs-shHAX1. Absorbance at 254 nm. (c, d) Western blot analysis of the protein levels in HUVECs-NC and HUVECs-shHAX1 (*p < 0.05, **p < 0.01, p < 0.001, Student's t-test). (e) KEGG pathway enrichment analysis of the mass spectrometry results. (f) Schematic diagram of HAX1 affecting eIF4F. (g) Co-immunoprecipitation analysis between HAX1 and eIF4E in HUVECs. (h) Immunofluorescence of eIF4G and eIF4E in HUVECs, bar: 50 �m

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Extracell Vesicles