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Knock-out of ADCY6 cause heart defects in zebrafish. a Bright-field images showing the morphology of 2 dpf uninjected, scramble, adcy6a and adcy6b F0 crispant zebrafish. Scale bars, 0.5 mm. b mRNA expression analysis of myl7 in 2dpf crispant hearts. Upper panels of (i) and (ii) imaged from control larvae. Lower panels imaged from (i) adcy6a and (ii) adcy6b crispants. Scale bars, 50 µm. c, d Quantification of cardiac defects observed in 2 dpf cadcy6a and dadcy6b crispants upon mRNA expression analysis of myl7. e–f Proportion of heart phenotypes observed in eadcy6a and fadcy6b crispants. Numbers central within bars indicate number of larvae in each classification. g–h Normalized heart rate measurements in beats per minute (bpm) analyzed at 2 dpf in gadcy6a and hadcy6b crispants. Two-way ANOVA (c, d), ordinary one-way ANOVA (g) and Kruskal–Wallis test (h) used for statistical analysis. Asterisk indicate P values: ***p < 0.001. n.s.: not significant
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