IMAGE

Fig. 5

ID

ZDB-IMAGE-241031-15

Genes

myl7

Publication

Baird et al., 2024 - Rare homozygous cilia gene variants identified in consanguineous congenital heart disease patients

All Figures

Figures for Baird et al., 2024

Image

Figure Caption

Fig. 5

Knock-out of ADCY6 cause heart defects in zebrafish. a Bright-field images showing the morphology of 2 dpf uninjected, scramble, adcy6a and adcy6b F0 crispant zebrafish. Scale bars, 0.5 mm. b mRNA expression analysis of myl7 in 2dpf crispant hearts. Upper panels of (i) and (ii) imaged from control larvae. Lower panels imaged from (i) adcy6a and (ii) adcy6b crispants. Scale bars, 50 µm. c, d Quantification of cardiac defects observed in 2 dpf cadcy6a and dadcy6b crispants upon mRNA expression analysis of myl7. e–f Proportion of heart phenotypes observed in eadcy6a and fadcy6b crispants. Numbers central within bars indicate number of larvae in each classification. g–h Normalized heart rate measurements in beats per minute (bpm) analyzed at 2 dpf in gadcy6a and hadcy6b crispants. Two-way ANOVA (c, d), ordinary one-way ANOVA (g) and Kruskal–Wallis test (h) used for statistical analysis. Asterisk indicate P values: ***p < 0.001. n.s.: not significant

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Genet.