Figure 5—figure supplement 1.

Calcineurin (CaN) repression increases the proportion of duct proliferating cells.

(1–3) Barplot representing the number of GFP+ ductal cells which in pancreatic tail of Tg(ins:NTR-P2A-mCherry); Tg(nkx6.1:GFP) larvae at 4 dpf for the Notch test. (1) Mild Notch inhibition with LY411575 5 µM and (3) stronger Notch inhibition with LY411575 15 µM. The black dots represent the control; gray Cyclosporin A (CsA) treatment; blue LY411575; and purple combination of LY411575 and CsA. Data are presented as mean values ± standard deviation (SD). Two-way ANOVA test with Tukey’s multiple comparisons test, *p-value <0.05; **p-value <0.005; ns = non-significant. (2) Z-plan pictures of Tg (nkx6.1:GFP); Tg (ins:NTR*-mCherry) larvae that were treated at 3 dpf with both CsA 1 µM and LY411575 5 µM. At 4 dpf, the larvae were exposed to 5‐ethynyl‐2′‐deoxyuridine (EdU) 4 mM during 8 hr, before analysis at 6 dpf.