Figure 4—figure supplement 1.

Calcineurin (CaN) repression potentializes the effect of Notch inhibition on beta cell formation.

(1) Experimental design for Notch inhibition test in non-ablated condition. Larvae were treated concomitantly with LY411575 (Notch inhibitor) and FK506 from 3 to 4 dpf and were fixed and analyzed at 5 dpf. (2) Barplot representing the mean number of mCherry+ beta cells in the pancreatic tail of Tg(ins:NTR-P2A-mCherry); Tg(nkx6.1:GFP) larvae at 5 dpf. The black dots represent the control; gray FK506 treatment; blue LY411575; and purple combination of LY411575 and FK506. Data are presented as mean values ± standard deviation (SD). Two-way ANOVA with Tukey multiple comparison test, *p-value <0.05,**p-value <0.005, ****p-value <0.00005. (3) Experimental design for Notch inhibition test in non-ablated condition. Larvae were heat-shocked and then directly treated with LY411575 (Notch inhibitor) from 3 to 4 dpf and were fixed and analyzed at 6 dpf. (4) Barplot representing the mean number of mCherry+ beta cells in the pancreatic tail of Tg(hsp70:CaN^CA); Tg(ins:NTR-P2A-mCherry); Tg(nkx6.1:GFP) larvae at 5 dpf. The black dots represent the control; gray heat-shock; blue LY411575; and purple combination of LY411575 and heat-shock. Data are presented as mean values ± SD. Two-way ANOVA with Tukey multiple comparison test, ****p-value <0.00005. (5) Barplot representing the mean number of mCherry+ beta cells in the pancreatic tail of Tg(ins:NTR-P2A-mCherry); Tg(nkx6.1:GFP) larvae at 6 dpf after Notch inhibition (LY411575), CaN inhibition (Cyclosporin A, CsA), and NFATc inhibition (CHIR99021). Data are presented as mean values ± SD. Two-way ANOVA with Tukey multiple comparison test, *p-value <0.05, **p-value <0.005, ****p-value <0.00005.