Fig. 6

Organ left-right patterning defects in embryos injected with cdon MO at the 256-cell stage (A) Embryos injected with cdon MO at the 256-cell stage were found to cause liver LR defects. a1, normal liver in Tg(fabp10:GFP) transgenic controls (96.6%, n = 90); a2, normal liver in Tg(fabp10:GFP) transgenic embryos injected with cdon MO at the 256-cell stage (62.2%, n = 111); a3, liver bifida in Tg(fabp10:GFP) transgenic embryos injected with cdon MO at the 256-cell stage (27.0%, n = 111); a4, reversed liver in Tg(fabp10:GFP) transgenic embryos injected with cdon MO at the 256-cell stage (10.8%, n = 111). a5, normal liver in wild-type controls (95.1%, n = 103); a6-a8, wild-type embryos injected with cdon MO at the 256-cell stage were examined for liver laterality at 4 dpf by WISH against fabp10 probe (n = 108). (B) Percentages of normal liver, liver bifida, and reversed liver in control embryos and embryos injected with cdon MO at the 256-cell stage. Embryos injected with cdon MO at the 256-cell stage show a statistically significant difference compared to controls. (C) Heart morphogenesis in Tg(cmcl2:GFP) transgenic embryos and wild-type embryos injected with cdon MO at the 256-cell stage. c1, normal-loop in Tg(cmcl2:GFP) transgenic controls (97.5%, n = 80); c2, normal-loop in Tg(cmcl2:GFP) transgenic embryos injected with cdon MO at the 256-cell stage (50.5%, n = 93); c3, no loop in Tg(cmcl2:GFP) transgenic embryos injected with cdon MO at the 256-cell stage (34.4%, n = 93); c4, reversed-loop in Tg(cmcl2:GFP) transgenic embryos injected with cdon MO at the 256-cell stage (15.1%, n = 93); c5, normal-loop in wild type controls (97.2%, n = 72); c6-c8, wild-type embryos injected with cdon MO at 256-cell stage were examined for cardiac looping at 72 hpf by WISH against my17 (n = 110). The blue dashed line indicates the atrial edge. (D) Percentages of normal looping, no looping, and reversed looping of the heart in all the embryos injected with or without cdon MO at the 256-cell stage. Embryos injected with cdon MO at the 256-cell stage show a statistically significant difference compared to controls. (E,F) Expression of Nodal/spaw in controls (n = 79) and embryos injected with cdon MO at the 256-cell stage (n = 92). (G,H) Expression of lefty1 in controls (n = 86) and embryos injected with cdon MO at the 256-cell stage (n = 91). (I,J) Expression of lefty2 in controls (n = 71) and embryos injected with cdon MO at the 256-cell stage (n = 87). Statistical analysis was performed using Student?s t-test. ?**? p < 0.01, ?***? p < 0.001, ?****? p < 0.0001. Notice: in ?B? and ?D?, all the transgenic embryos and wild-type embryos were used together to calculate the percentage. ?Control? refers to wild-type embryos that were not injected with cdon MO.