Fig. 4
- ID
- ZDB-FIG-240906-33
- Publication
- Deng et al., 2024 - Cdon is essential for organ left-right patterning by regulating dorsal forerunner cells clustering and Kupffer's vesicle morphogenesis
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Organ LR patterning defects in cdon?/?embryos (A) CRISPR/Cas9 target was showed. The sequence in the exon 3 of cdon gene was chosen as the target (yellow highlighted). (B) Sequencing results for the genomic DNA in WT embryos, cdon+/? embryos and cdon?/? embryos. (C) Nucleotide sequences highlighted in yellow are CRISPR/Cas9 targets. In the cdon mutant, the sequence ?AAGGGC? was changed to ?TTGATGAATGGGG?. Cdon mutant harbors a frameshift mutation that is predicted to result in the production of truncated Cdon protein (104 amino acids). (D) Cdon?/? embryos were found to display liver LR defects using WISH. d1, left-sided liver in controls (95.5%, n = 111); d2, left-sided liver in cdon?/? embryos (71.2%, n = 118); d3, liver bifida in cdon?/? embryos (17.8%, n = 118); d4, right-sided liver in cdon?/? embryos (11.0%, n = 118). (E) Percentages of left-sided liver, liver bifida, and right-sided liver in cdon?/? embryos (n = 118), controls (n = 111) and cdon?/? embryos injected with cdon mRNA (n = 89), cdon?/? embryos show a statistically significant difference compared to controls, and cdon?/? embryos injected with cdon mRNA show a statistically significant difference compared to cdon?/? embryos. (F) Cdon?/? embryos displayed heart LR defects. f1, normal-loop heart in controls (97.5%, n = 122); f2, normal-loop heart in cdon?/? embryos (72.7%, n = 128); f3, no loop heart in cdon?/?embryos (16.4%, n = 128); f4, reversed loop heart in cdon?/? embryos (10.9%, n = 128). The blue dashed line indicates the atrial edge. (G) Percentages of normal looping, no looping, and reversed looping of the heart in cdon?/? embryos (n = 128), controls (n = 122) and cdon?/? embryos injected with cdon mRNA (n = 90), cdon?/? embryos show a statistically significant difference compared to controls, and cdon?/? embryos injected with cdon mRNA show a statistically significant difference compared to cdon?/? embryos. Statistical analysis was performed using Student?s t-test. ?*? p < 0.05, ?**? p < 0.01, ?***? p < 0.001. Notice: ?control? refers to wild-type embryos. |