FIGURE

Fig. 8

ID

ZDB-FIG-240905-8

Publication

Chen et al., 2024 - NEDD8 enhances Hippo signaling by mediating YAP1 neddylation

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Fig. 8

Zebrafish nedd8 enhances yap1 transcriptional activity. A, 8xGTIIC-Luc reporter activity in Myc empty vector (200 ng) or Myc-yap1 (200 ng) transfected HEK293T cells with or without HA-nedd8 for 36 h. B, 8xGTIIC-Luc reporter activity in Myc empty vector (200 ng) or Myc-yap1 (200 ng) transfected HEK293T cells with or without 1 ?M MLN4924 treatment for 24 h. C, 8xGTIIC-Luc reporter activity in Myc empty vector (200 ng) or Myc-yap1 (200 ng) transfected HEK293T cells with or without HA-uba3 and HA-ubc12 for 36 h. D, 8xGTIIC-Luc reporter activity in Myc empty vector (200 ng) or Myc-yap1 (200 ng) transfected HEK293T cells with or without HA-senp8 for 36 h. E, 8xGTIIC-Luc reporter activity in Myc empty vector (200 ng) or Myc-yap1 (200 ng) transfected KGN cells with or without HA-nedd8 for 36 h. F, 8xGTIIC-Luc reporter activity in Myc empty vector (200 ng) or Myc-yap1 (200 ng)-transfected KGN cells with or without MLN4924 (1 ?M) treatment for 24 h. Four separate experiments were performed, with each measurement performed at least three times. Data are expressed as mean � SD. (unpaired two-tailed Student's t test). ?p < 0.05, ??p < 0.01, and ???p < 0.001. ns, not significant; YAP1, yes-associated protein 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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