FIGURE

Fig. 4

ID

ZDB-FIG-240905-4

Publication

Chen et al., 2024 - NEDD8 enhances Hippo signaling by mediating YAP1 neddylation

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Fig. 4

Neddylation of YAP1 promotes its nuclear translocation. A and B, immunofluorescence staining of endogenous NEDD8 and MST1 (A), NEDD8 and YAP1 (B) expression, and localization in the rabbit ovary. The nucleus was stained blue with DAPI (the scale bar represents 100 ?m). C, confocal microscopy image of cotransfection with EGFP-NEDD8 and mCherry-YAP1 plasmids in H1299 cells, the nucleus was stained blue with DAPI (the scale bar represents 10 ?m). D, Western blot analysis of the indicated protein levels of YAP1, p-YAP1, NEDD8, CDKN1A, MST1, and CTGF in rabbit GCs treated with or without MLN4924 (1 ?M) for 0 to 48 h. E, quantification of the above proteins expression level. F, neddylation of YAP1 is detected by immunoprecipitation and Western blot in HEK293T cells in response to NEDD8 overexpression using Ni-NTA agarose beads. G, Western blot analysis and the quantification of the indicated protein in nuclear (Nuc) versus cytoplasmic (Cyt) fractionation from rabbit GCs with or without MLN4924 (1 ?M) treatment for 24 h. Data are represented as means � SD, n = 3 experiments, ?p < 0.05, ??p < 0.01, and ???p < 0.001. Cyt, cytoplasm; DAPI, 4?, 6-diamidino-2-phenylindole; GC, granulosa cell; IB, immunoblotting; IP, immunoprecipitation; ns, not significant; Nuc, nucleus; TCL, total cell lysate; YAP1, yes-associated protein 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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