Fig. 7
Disruption of nedd8 in zebrafish results in the inhibition of yap1 signaling. A, sequence information diagram of nedd8-null zebrafish. A 4-bp nucleotide deletion (5′-GAGC-3′) occurred in exon 3 of the nedd8 deletion mutant, resulting in a reading frame shift. B–D, qPCR analysis of yap1 target genes that are either positively or negatively regulated at the mRNA level in WT and nedd8-null zebrafish ovary (B), brain (C), and muscle (D). β-actin was used as an internal control. E, Western blot analysis of GAPDH, birc5, ctgf, yap1, and nedd8 protein levels in brain of nedd8+/+ and nedd8−/− zebrafish. GAPDH was used as an internal control. The red numbers represent the quantified results of the protein. Data are representative of three independent experiments (mean ± SD of three technical replicates). Unpaired two-tailed Student's t test was performed. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ns, not significant; qPCR, quantitative real-time PCR; YAP1, yes-associated protein 1. |