FIGURE

Fig. 7

ID

ZDB-FIG-240905-7

Publication

Chen et al., 2024 - NEDD8 enhances Hippo signaling by mediating YAP1 neddylation

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Fig. 7

Disruption of nedd8 in zebrafish results in the inhibition of yap1 signaling. A, sequence information diagram of nedd8-null zebrafish. A 4-bp nucleotide deletion (5?-GAGC-3?) occurred in exon 3 of the nedd8 deletion mutant, resulting in a reading frame shift. B?D, qPCR analysis of yap1 target genes that are either positively or negatively regulated at the mRNA level in WT and nedd8-null zebrafish ovary (B), brain (C), and muscle (D). ?-actin was used as an internal control. E, Western blot analysis of GAPDH, birc5, ctgf, yap1, and nedd8 protein levels in brain of nedd8+/+ and nedd8?/? zebrafish. GAPDH was used as an internal control. The red numbers represent the quantified results of the protein. Data are representative of three independent experiments (mean � SD of three technical replicates). Unpaired two-tailed Student's t test was performed. ?p < 0.05, ??p < 0.01, and ???p < 0.001. ns, not significant; qPCR, quantitative real-time PCR; YAP1, yes-associated protein 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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