FIGURE

Fig. 5

ID

ZDB-FIG-240904-31

Publication

Anticevic et al., 2024 - Tyrosyl-DNA phosphodiesterase 2 (Tdp2) repairs DNA-protein crosslinks and protects against double strand breaks in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Silencing of tdp2b causes accumulation of Top2-DPCs, Ku80-DPCs and DSBs. (A) Dot blots and corresponding quantifications normalized to WT embryos showing (A) Top2-DPCs (n = 2), (B) Ku80-DPCs (n = 2), and (C) histone H3-DPCs (n = 3) with corresponding DNA loading controls, after tdp2b silencing (tdp2bMO) or Tdp2b overexpression in tdp2b-silenced embryos (tdp2bMO+2b). (D) Western blot analysis of γH2AX levels in WT embryos (WT), tdp2a-silenced (tdp2aMO), tdp2b-silenced (tdp2bMO); tdp2a- and tdp2b-silenced (tdp2a/2bMO) and in embryos where Tdp2b was overexpressed in tdp2bMO (tdp2bMO+2b). Tubulin was used as a loading control. WT embryos treated with formaldehyde (10 mM, 30 min) or etoposide (50 μM, 1 h) were used as a positive control for DSB induction. (E) Quantification of (D). Data show the mean fold change compared to WT ± SEM (n = 3). Statistical significance was determined using an unpaired Student’s t-test (* (p < 0.05), ** (p < 0.01) and *** (p < 0.001)).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol