Fig. 1

Phylogenetic analysis and domain organization of tyrosyl-DNA phosphodiesterase 2 in humans and zebrafish. (A) Phylogenetic tree of tyrosyl-DNA phosphodiesterase 2 (TDP2). Vertebrate orthologs are shown in blue with an additional cluster of Tdp2 co-orthologs in fish is shown in light blue. Two clusters of invertebrate orthologs are shown in green, algae in light green, plant orthologs in red, fungi in brown, and bacterial cluster in grey. Phylogenetic analysis was performed using the Maximum Likelihood method. (B) Domain structures of human and zebrafish tyrosyl-DNA phosphodiesterase 2 (UBA - ubiquitin-associated domain; EEP-exonuclease/endonuclease/phosphodiesterase catalytic domain). Conserved catalytic motifs bearing catalytic residues are shown in blue and DNA binding sites in Tdp2b are shown in orange.