Fig. 2

Syntenic analysis of human and zebrafish TDP2 and mRNA expression patterns in embryos and adults. (A) Synteny analysis of zebrafish and human TDP2 genes. The schematic shows the chromosomal positions of the zebrafish and human TDP2 genes as determined using the Genomics database. The numbers next to the gene names indicate their respective position in megabase pairs (Mbp) on the respective chromosome. (B) mRNA expression profiles of tdp2a and tdp2b during zebrafish embryonic development from 6 h post-fertilization (6 hpf) to 5 days post-fertilization (5 dpf), normalized to the housekeeping gene ATP synthase peripheral stalk (atp50). (C) Tissue expression pattern of tdp2a and (D) tdp2b in adult zebrafish: brain, liver, kidney, intestine and gonads. Statistically significant differences (*p < 0.05, **p < 0.01, ***p < 0.001) were determined by unpaired t-test. Data are presented as MNE (mean normalized expression) ± SEM (n = 3), normalized to the housekeeping gene ATP synthase peripheral stalk subunit (atp50).