Fig. 5

PATCH-CLAMP RECORDINGS OF CLC-1-LIKE CURRENTS IN ZEBRAFISH PRIMARY CULTURES OF MYOTUBES A, close image of a zebrafish primary culture of myotubes. Extracts from myotubes were analysed by western blot using antibodies against clc-1a and clc-1b detecting both proteins in the cultures. B, representative current trace of the elicited activity. Whole-cell currents were elicited by injecting voltage pulses as described in oocytes. C, steady-state I–V relationship of the myotubular clc-1-like current (N = 6). Data points represent means ± SD. D, relative clc-1-like current before (Pre) and after the application of 100 µm and 1 mm 9-AC (N = 3). Ordinary one-way ANOVA with Tukey's multiple comparisons test was performed. While no significant effect was detected after the application of 100 µm 9-AC (P = 0.86), 1 mm 9-AC significantly altered clc-1-like currents (P = 0.03). Both current characteristics and 9-AC reduced sensitivity resemble the clc-1 orthologue current properties previously shown in Xenopus oocyte recordings.