Fig. 4

Defining the node-type composition of the intrahepatic biliary network. Computational network analysis revealed the intrahepatic biliary network could be defined as a combination of different types of nodes. (A) Schematics of node-subtypes existing in the intrahepatic biliary network. (B) Ratio of node sub-type distribution in the intrahepatic biliary network in wild-type larvae at 5 dpf. X-axis labels denote the number of branches connected to the node (#W), how many of those branches are node-node connections (#N), and how many of those branches are node-endpoint connections (#E). In the schematics, node (white circle), endpoint (green circle), node-node (red bar) and node-endpoint (yellow bar) connections indicate each node sub-type compositions. (C) Ratio of 3-way node sub-types in wild-type (WT) is plotted separately. The 3W2N1E node always exists significantly higher than the 3W1N2E node in WT. (D) Ratio of 4-way node sub-types in WT is plotted separately. Similar to the distribution of 3-way nodes, the 4W3N1E node always exists with the highest ratio in WT while the 4W1N3E node is always lower than other node sub-types. (E) Ratio of 5-way node sub-types in WT is plotted separately. The 5W1N4E node always exists at the lowest ratio in WT. (F) Ratio of 3-way node sub-types in lri31 (IZ-1.6) mutant larvae at 5 dpf. The ratio difference between 3W2N1E and 3W1N2E is lost in lri31 mutant larvae. (G) Ratio of 4-way node sub-types in lri26 (HZ-1.4) mutant larvae at 5 dpf. The relative ratio of the 4W1N3E node was increased in lri26 mutant larvae. (H) Ratio of 5-way node sub-types in lri17 (CD-1.4) mutant larvae at 5 dpf. The relative ratio of the 5W1N4E node is increased in lri17 mutant larvae. These data indicate the distribution of node sub-types is regulated in part by these mutations. Bars with a shared letter indicate the difference is not statistically significant.