Fig. 2

Clustered heat map-based classification of recovered mutants. Clustered heat map based on the structural sub-parameters of the intrahepatic biliary network in recovered mutant larvae at 5 dpf. All sub-parameters are normalized to the average value of the corresponding sub-parameters in the wild-type dataset. Clustering was done with the hierarchical clustering algorithm. Red indicates a higher value than wild-type, and blue indicates a lower value than wild-type. Values represent the average for each specimen dataset. The x-axis of the dendrogram indicates the relative similarity of each structural sub-parameter to other sub-parameters. The shorter the line between two sub-parameters, the more closely related they are. Similarly, the y-axis indicates the relative similarity of each mutant phenotype to other mutant phenotypes. Classes are denoted by the color of the y-axis dendrogram: Wild-type (Black), Class Ia (Dark Blue), Class Ib (Light Blue), Class II (Red), and Class III (Green). Classification was unbiasedly based on network structural sub-parameters of the intrahepatic biliary network.