Fig. 3

Principal component analysis (PCA) dimensionality reduction analysis-based mutant classification. (A) The PCA dimensionality reduction plot of wild-type and recovered mutants based on the mean values of biliary network structural sub-parameters within the allele indicates the similarity of phenotypes. Colors denote the defined class of that specimen group: Wild-type (Black), Class Ia (Dark Blue), Class Ib (Light Blue), Class II (Red), and Class III (Green). Proximity of the data points indicates the relative similarity of the recovered mutants; the closer the points, the more closely related the phenotypes are. (B) The PCA plot of all individual larvae of wild-type and recovered mutants analyzed in this study based on biliary network structural sub-parameters. The plot is labeled based on the assigned classes: Wild-type (Black), Class Ia (Dark Blue), Class Ib (Light Blue), Class II (Red), and Class III (Green). The same plots showing only the data from each class are shown separately. Each class of mutants was distributed in a pattern that partially overlapped but was still clearly distinct.