Fig. 7

ROS and acrolein accumulation in Smg9-deficient zebrafish.

a, c Representative flow cytometry histograms of CellROX fluorescence in smg9^+/+ and smg9^oi7/oi7 zebrafish at 3 mpf (a) and 6 mpf (c). b, d Mean fluorescence intensity of CellROX Deep Red in the brains of smg9^+/+ and smg9^oi7/oi7 zebrafish at 3 mpf (b) and 6 mpf (d). e, g Representative images of AcroleinRED staining of smg9^+/+ and smg9^oi7/oi7 zebrafish brains at 3 mpf (e) and 6 mpf (g). f, h Relative mean fluorescence intensities of smg9^+/+ and smg9^oi7/oi7 zebrafish brains at 3 mpf (f) and 6 mpf (h). i, j Representative images of transmission electron microscopy of smg9^+/+ and smg9^oi7/oi7 zebrafish brains at 6 mpf. The red arrowhead and red arrow indicate normal and damaged mitochondria, respectively. N Nucleus. Scale bar: 5 µm in the upper panel and 1 µm in the lower panel (i). The density of damaged mitochondria within a defined area (0.25 mm²) in the telencephalon region of six individual zebrafish brains (j). Error bars indicate SD. *P < 0.05, ** P < 0.01, and *** P < 0.001. ns: not significant.