Fig. 8

Smox is required for brain and heart defects in Smg9-deficient zebrafish.

a Representative image of hematoxylin and eosin staining of smg9^+/+ and smg9^oi7/oi7 larvae after treatment with or without MDL72527. Scale bar: 50 µm. b Quantification of the telencephalon area of smg9^+/+ and smg9^oi7/oi7 larvae after treatment with or without MDL72527. c Representative images of immunostaining for PCNA (red), S100β (green), and DAPI (blue) for smg9^+/+ and smg9^oi7/oi7 larvae after treatment with or without MDL72527. Scale bar: 50 µm. d Quantification of PCNA/S100β double-positive radial glial cells among DAPI-positive cells in the telencephalon of smg9^+/+ and smg9^oi7/oi7 larvae after treatment with or without MDL72527. e Representative images of hematoxylin and eosin staining of smg9^+/+ and smg9^oi7/oi7 larvae after treatment with or without N-acetylcysteine (NAC). Scale bar: 50 µm. f Quantification of the telencephalon area of smg9^+/+ and smg9^oi7/oi7 larvae after treatment with or without NAC. Scale bar: 50 µm. g Representative images of immunostaining for PCNA (red), S100β (green), and DAPI (blue) for smg9^+/+ and smg9^oi7/oi7 larvae after treatment with or without NAC. Scale bar: 50 µm. h Quantification of PCNA/S100β double-positive radial glial cells among DAPI-positive cells in the telencephalon of smg9^+/+ and smg9^oi7/oi7 larvae, after treatment with or without NAC. i Representative dynamic pixel change patterns of the heart showing cardiac contractions of smg9^+/+ and smg9^oi7/oi7 zebrafish after treatment with or without MDL72527. j Quantification of cardiac contractions in smg9^+/+ and smg9^oi7/oi7 zebrafish larvae after treatment with or without MDL72527. k Representative dynamic pixel change patterns of the heart showing cardiac contractions of smg9^+/+ and smg9^oi7/oi7 zebrafish after treatment with or without NAC. l Quantification of cardiac contractions in smg9^+/+ and smg9^oi7/oi7 zebrafish larvae after treatment with or without NAC. m Representative images of hematoxylin and eosin staining of smg9^+/+ and smg9^oi7/oi7 larvae injected with control (con-in) or smox morpholino (MO-in). Scale bar: 50 µm. n Quantification of the telencephalon area of smg9^+/+ and smg9^oi7/oi7 larvae injected with control (con-in) or smox morpholinos (MO-in). o Representative images of immunostaining with PCNA (red), S100β (green), and DAPI (blue) for smg9^+/+ and smg9^oi7/oi7 larvae injected with control (con-in) or smox morpholino (MO-in). Scale bar: 50 µm. p Quantification of PCNA/S100β double-positive radial glial cells among DAPI-positive cells in the telencephalon of smg9^+/+ and smg9^oi7/oi7 larvae injected with control (con-in) or smox morpholino (MO-in). q Representative dynamic pixel change patterns of the heart showing cardiac contraction of smg9^+/+ and smg9^oi7/oi7 zebrafish injected with control (con-in) or smox morpholino (MO-in). r Quantification of cardiac contractions in smg9^+/+ and smg9^oi7/oi7 zebrafish larvae injected with control (con-in) or smox morpholino (MO-in). Error bars indicate SD. *P < 0.05, ** P < 0.01, and *** P < 0.001. ns: not significant.