FIGURE

Fig. 3

ID

ZDB-FIG-240529-52

Publication

Sun et al., 2024 - ptx3a⁺ fibroblast/epicardial cells provide a transient macrophage niche to promote heart regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

ptx3a+ cells associate with reparative macrophages (A) Histological visualization of ptx3a+ cells in hearts after injury at 0, 3, 7, and 30 dpa. White dashed line indicates amputation plane. n = 6–8 animals. Scale bar, 50 μm. (B) Visualization of ptx3a:EGFP+ cells with mpeg+ macrophages at 7 dpa in the injury site of ptx3a:mCherry;mpeg:EGFP ventricles. Dashed rectangle area is enlarged. Arrows represent mpeg+ macrophages associated with EGFP+ cells. n = 6. Scale bar, 50 μm. (C) The correlation of ptx3a+ cells with cxcr4b expression assessed by cxcr4b antibody staining in injured ventricles of ptx3a:EGFP animals at 7 dpa. Dashed rectangle is enlarged. Arrows represent cxcr4b+ macrophages associated with EGFP+ cells. n = 7. Scale bar, 50 μm. (D) Visualization of ptx3a:EGFP+ cells with tnfa+ macrophages at 7 dpa in the injury site of ptx3a:mCherry;tnfa:EGFP ventricles. Dashed rectangle area is enlarged. Arrows represent tnfa+ macrophages not associated with EGFP+ cells. n = 6. Scale bar, 50 μm. (E) The spatial correlation of macrophages and ptx3a+ cells are quantified by the distance of these cells with three situations: (1) macrophages are surrounded by ptx3a+ cells; (2) macrophages close to ptx3a+ cells within 1 μm except situation 1, and (3) macrophages away from ptx3a+ cells by more than 1 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.