FIGURE

Fig. 2

ID

ZDB-FIG-240528-75

Publication

Ruparelia et al., 2024 - Atrogin-1 promotes muscle homeostasis by regulating levels of endoplasmic reticulum chaperone BiP

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Fig. 2

Atrogin-1 deficiency results in impaired membrane integrity and apoptosis. Live images of methyl cellulose?treated 6 dpf atrogin-1+/+ (A?C) and atrogin-1?/? (D?F) on the (Tg(actc1b:Lifeact-GFP);Tg(actc1b:CAAX-mCherry) background, whereby the actin filaments within the muscle fibers are labeled with GFP and membrane and t-tubules with mCherry. While muscle cells in the atrogin-1+/+ wild-type larvae span the entire length of the somite, with the sarcolemma fully surrounding each cell, atrogin-1?/? mutants display detached muscle fibers that are surrounded with irregular sections of sarcolemma, along with presence of abnormal sarcolemma vacuoles. Live images of methyl cellulose?treated 6 dpf atrogin-1+/+ (G?I) and atrogin-1?/? (J?L) larvae injected with Evans blue dye. atrogin-1?/? mutants displayed an accumulation of the dye, which is absent in atrogin-1+/+ larvae. TUNEL staining on 3 dpf methyl cellulose?treated atrogin-1+/+ (M?O) and atrogin-1?/? (P?R) larvae, with the latter displaying TUNEL in areas where the muscle cell had detached. Scale bar: 100 ?m (A?F); 300 ?m (G?R).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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