Investigating the impact of cdc27 knockout on pharyngeal pouches, neural crest cells (NCCs), and pharyngeal cartilage development in zebrafish embryos. This figure presents a series of in situ hybridization (ISH) and fluorescence imaging analyses to assess the developmental changes in cdc27−/− embryos compared to control siblings. (A–D) ISH results with crestin and foxd3 probes at 24 h post-fertilization (hpf) and 28 hpf. The staining patterns for crestin and foxd3, markers for NCCs, show no noticeable differences between mutant embryos and their control siblings. (E,F) Fluorescence imaging of sox10-labeled NCCs in the pharyngeal arch region, marked by a white dotted box and enlarged in the solid white box. Green fluorescence signals indicating NCCs are similar in both cdc27−/− embryos and siblings. (G,H) ISH with the dlx2a probe at 30 hpf. Expression of dlx2a, a gene involved in craniofacial development, appears similar in both mutant and control embryos. (I–L) ISH with tbx1 and fgf3 probes at 48 hpf. The results show no significant differences in the segmentation and number of pharyngeal pouches between cdc27−/− embryos and siblings. (M–P) ISH with the barx1 probe at 48 hpf. No significant variation is observed in barx1 expression between mutant and control embryos. (Q–X) ISH with sox9a and col2a1a probes at 72 hpf. While sox9a expression is notably reduced in mutants, the expression of col2a1a, a marker for cartilage, is absent in the hypopharyngeal arches of the mutant embryos compared to controls, indicating a significant disruption in cartilage development.
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