Figure 5

Phenotypic comparison between cdc27^−/− mutants and wild-type zebrafish embryos from 1 to 5 days post-fertilization (dpf). This figure showcases the developmental progression and phenotypic variations observed in cdc27^−/− mutant embryos compared to wild-type embryos from 1 dpf to 5 dpf. At 1 dpf (A,B), there are no significant phenotypic differences between the two groups. By 2 dpf (C,D), cdc27^−/− embryos exhibit microcephaly, microphthalmia, and an abnormally curved spine, yet without noticeable abnormalities in the pharyngeal arch. At 3 dpf (E,F), the mutant embryos begin to show more pronounced phenotypic changes, including the absence of mandible structure formation, exacerbated spinal deformity, and the emergence of cardiac edema. These developmental anomalies, particularly the abnormal mandible development, persist in the mutant embryos at 4 dpf (G,H) and 5 dpf (I,J), highlighting the progressive nature of the craniofacial and skeletal deformities in cdc27^−/− mutants.