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Fig. 2

ID

ZDB-FIG-240509-21

Publication

Zhang et al., 2024 - ERK-activated CK-2 triggers blastema formation during appendage regeneration

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Fig. 2

Screening of ERK-binding proteins required for leg regeneration. (A) Schematic of GST pull-down combined with LC-MS/MS for identifying ERK targets. The beads immobilized GST-ERKs were incubated with American cockroach leg extracts. After incubation and washing, the binding proteins were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. P indicates ERK-binding proteins. (B) List of ERK associated protein participated in embryonic development. (C) Screening the binding proteins of ERK from (B) for leg regeneration by in vivo RNAi. RNAi knockdown of CK-2 severely inhibits leg regeneration (red frame).The red dashed line represents the amputation plane. Scale bars, 1 mm. (D) Statistical analysis for the effect of the genes from (C) on the leg regeneration. The regenerated leg length was measured by using ImageJ. n represents the number of regenerated legs. Student’s t test. m/z, mass/charge ratio.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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