PUBLICATION
ERK-activated CK-2 triggers blastema formation during appendage regeneration
- Authors
- Zhang, X.S., Wei, L., Zhang, W., Zhang, F.X., Li, L., Li, L., Wen, Y., Zhang, J.H., Liu, S., Yuan, D., Liu, Y., Ren, C., Li, S.
- ID
- ZDB-PUB-240321-3
- Date
- 2024
- Source
- Science advances 10: eadk8331 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Signal Transduction/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Wound Healing
- Regeneration*/genetics
- Animals
- Zebrafish*/metabolism
- PubMed
- 38507478 Full text @ Sci Adv
Citation
Zhang, X.S., Wei, L., Zhang, W., Zhang, F.X., Li, L., Li, L., Wen, Y., Zhang, J.H., Liu, S., Yuan, D., Liu, Y., Ren, C., Li, S. (2024) ERK-activated CK-2 triggers blastema formation during appendage regeneration. Science advances. 10:eadk8331.
Abstract
Appendage regeneration relies on the formation of blastema, a heterogeneous cellular structure formed at the injury site. However, little is known about the early injury-activated signaling pathways that trigger blastema formation during appendage regeneration. Here, we provide compelling evidence that the extracellular signal-regulated kinase (ERK)-activated casein kinase 2 (CK-2), which has not been previously implicated in appendage regeneration, triggers blastema formation during leg regeneration in the American cockroach, Periplaneta americana. After amputation, CK-2 undergoes rapid activation through ERK-induced phosphorylation within blastema cells. RNAi knockdown of CK-2 severely impairs blastema formation by repressing cell proliferation through down-regulating mitosis-related genes. Evolutionarily, the regenerative role of CK-2 is conserved in zebrafish caudal fin regeneration via promoting blastema cell proliferation. Together, we find and demonstrate that the ERK-activated CK-2 triggers blastema formation in both cockroach and zebrafish, helping explore initiation factors during appendage regeneration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping