FIGURE

Fig. 6

ID

ZDB-FIG-240306-65

Publication

Dennhag et al., 2024 - fhl2b mediates extraocular muscle protection in zebrafish models of muscular dystrophies and its ectopic expression ameliorates affected body muscles

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Fig. 6

fhl2b overexpression leads to upregulation of regenerative markers in dmd^−/− zebrafish larvae.

a Upset plot showing the intersection of DEGs between Tg(503unc:fhl2b-T2A-EGFP) vs sibling controls and dmd^−/−:Tg(503unc:fhl2b-T2A-EGFP) vs dmd^−/− larvae (Gene set D: DEGs caused by fhl2b overexpression in both comparisons; Gene set E: DEGs caused by fhl2b overexpression in healthy sibling control conditions alone; Gene set F: DEGs caused by fhl2b overexpression in the dmd^−/− disease condition). b Heatmap displaying the expression of DEGs in Gene set F. Red boxes indicate DEGs related to muscle regeneration. c Timelapse images of dmd^−/−;Tg(503unc:EGFP) and dmd^−/−;Tg(503unc:fhl2b-T2A-EGFP) larvae at 3.5, 4 and 4.5 dpf, respectively. Asterisks (*) indicate damaged somites. Area viewed is indicated in zebrafish illustration above. d Birefringence images following the same dmd^−/−;Tg(503unc:EGFP) and dmd^−/−;Tg(503unc:fhl2b-T2A-EGFP larvae at 3, 4 and 5 dpf. Asterisks (*) indicate damaged somites. Trunk region viewed is indicated in zebrafish illustration above. E Quantification of birefringence in damaged somites over time. Birefringence at 4 and 5 dpf was compared to 3 dpf in order to evaluate muscle regeneration progression over time. p = 0.012 at 4 dpf and p = 0.051 at 5 dpf. Statistical analysis in e, f: Two-sided Wald test with B/H-correction, k, l, o, p: Two-sided t-test with Welsh correction. Scale bar in c: 50 µm and d: 200 µm. Schematic images were adapted from https://www.biorender.com.

Expression Data

Genes:	flnca kdm6ba mmp14b ptgs2b rbfox1l
Fish:	dmd^{ta222a/ta222a}; umu34Tg/umu34Tg
Anatomical Term:	trunk
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dmd^{ta222a/ta222a} dmd^{ta222a/ta222a}; umu37Tg/umu37Tg dmd^{ta222a/ta222a}; umu34Tg/umu34Tg
Observed In:	trunk trunk musculature muscle tissue morphogenesis trunk musculature skeletal muscle tissue regeneration
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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