Fig 5

Treatment with MEndoB results in dose-dependent survival of up to 90% in a lethal mouse bacteremia model. Schematic representation of the experimental procedure (A). Survival rates of mice infected with S. aureus USA300 LAC strain treated with vehicle (PBS) or MEndoB at 0.1 mg/kg (gray), 1.0 mg/kg (pink), and 10.0 mg/kg (green) were monitored over 48 h post-treatment (n = 10 per group) (B). CFUs in the blood of treated animals were analyzed after 0, 1, 6, and 48 h (C). The limit of detection was 2.7 Log10 CFUs/mL (dotted line). The number of analyzed blood samples is shown in the table; only non-deceased animals with clinical scores permitting blood sampling were included in the analysis (no vehicle group beyond 6 h time point). TNF-α serum concentration in the blood of the mice infected with S. aureus USA300 LAC strain was analyzed 48 h post-treatment (D). For all graphs, error bars indicate the standard error of the mean. Statistical analysis performed for survival rates was a log-rank (Mantel-Cox) test (vehicle vs treatment groups) and an unpaired t-test at 48 h for blood CFU counts and TNF-α concentrations (****P < 0.0001, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05, and ns > 0.05).