Analysis of the impact of P. gingivalis OMVs on the neutrophils. (A) Representation of confocal image at 0 hpi with microinjection of PBS or 2.5 µg/µL P. gingivalis OMVs in that heart region of the body. Bright, Bright channel, Red, P. gingivalis OMVs; Green, neutrophils. Scale bar: 100 μm. (B) Image of neutrophils fluorescence at 2 hpi after treated with PBS or 2.5 µg/µL P. gingivalis OMVs. Live imaging was performed under a stereoscopic fluorescence microscope (SMZ800N) in the green channel. Scale bar: 0.5 mm. (C) Analysis results of neutrophils fluorescence according to picture taken in Fig. 4B. n = 15, each group being replicated thrice. The unit is pixel, Mann Whitney test was used to analyze the significant difference between groups. *p < 0.05. (D) Image of neutrophils fluorescence at 24 hpi after treated with PBS or 2.5 µg/µL P. gingivalis OMVs. Scale bar: 0.5 mm. (E) Analysis results of neutrophils fluorescence according to picture taken in Fig. 4D. n = 15, each group being replicated thrice. Mann Whitney test was used to analyze the significant difference between groups, **p < 0.01. (F) Image of neutrophils fluorescence at 48 hpi after treated with PBS or 2.5 µg/µL P. gingivalis OMVs. Scale bar: 0.5 mm. (G) Analysis results of neutrophils fluorescence according to picture taken in Fig. 4F. n = 15, each group being replicated thrice. Mann Whitney test was used to analyze the significant difference between groups, **p < 0.01
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