Fig. 4

Activity of FASN and mTOR is essential for compensatory cell growth in the developing periderm. (A,B) Immunostainings using anti-FASN antibody followed by confocal imaging (A) and fluorescence intensity quantification (B) in the embryos obtained from the Tg(cldnB:lynEGFP) line that are morphants for ?Np63 (?Np63 MO) or treated with PD168393, and fixed at 48 hpf. (C-F) Confocal scans (C,D) and graphical representation of the total, apical and basolateral area quantifications (E,F) in ?Np63 morphants (?Np63 MO) and PD168393-treated embryos upon FASN inhibition by Cerulenin (C,E) or mTOR inhibition by Rapamycin (D,F). Data are mean±s.e.m. (B) and median±interquartile range (E,F). ***P<0.001 [Mann?Whitney U test (B); Kruskal?Wallis test with Dunn's post hoc test (E,F)]. ns, not significant. Please note that only important paired comparisons are shown. For the entire pair-wise analyses please refer to Table S3. Scale bars: 20 µm.